Coding

Part:BBa_K2020026:Design

Designed by: Katja Schröder   Group: iGEM16_Aachen   (2016-10-13)


secretion tag for Saccharomyces cerevisiae


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence contained an illegal restriction site, which had to be corrected via PCR.


Source

It is the alpha mating factors secretion tag, which is naturally occurring in the Saccharomyces cerevisiae genome.

References